Crystallization and preliminary X‐ray diffraction analysis of the N‐terminal domain of human coronavirus OC43 nucleocapsid protein
Identifieur interne : 002841 ( Main/Exploration ); précédent : 002840; suivant : 002842Crystallization and preliminary X‐ray diffraction analysis of the N‐terminal domain of human coronavirus OC43 nucleocapsid protein
Auteurs : I-Jung Chen ; Chia-Cheng Chou ; Chia-Ling Liu ; Cheng-Chung Lee ; Lou-Sing Kan ; Ming-Hon HouSource :
- Acta Crystallographica Section F [ 1744-3091 ] ; 2010-06-01.
Abstract
The N‐terminal domain of nucleocapsid protein from human coronavirus OC43 (HCoV‐OC43 N‐NTD) mostly contains positively charged residues and has been identified as being responsible for RNA binding during ribonucleocapsid formation in the coronavirus. In this study, the crystallization and preliminary crystallographic analysis of HCoV‐OC43 N‐NTD (amino acids 58–195) with a molecular weight of 20 kDa are reported. HCoV‐OC43 N‐NTD was crystallized at 293 K using PEG 1500 as a precipitant and a 99.9% complete native data set was collected to 1.7 Å resolution at 100 K with an overall Rmerge of 5.0%. The crystals belonged to the hexagonal space group P65, with unit‐cell parameters a = 81.57, c = 42.87 Å. Solvent‐content calculations suggest that there is likely to be one subunit of N‐NTD in the asymmetric unit.
Url:
DOI: 10.1107/S1744309110017616
Affiliations:
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In this study, the crystallization and preliminary crystallographic analysis of HCoV‐OC43 N‐NTD (amino acids 58–195) with a molecular weight of 20 kDa are reported. HCoV‐OC43 N‐NTD was crystallized at 293 K using PEG 1500 as a precipitant and a 99.9% complete native data set was collected to 1.7 Å resolution at 100 K with an overall Rmerge of 5.0%. The crystals belonged to the hexagonal space group P65, with unit‐cell parameters a = 81.57, c = 42.87 Å. Solvent‐content calculations suggest that there is likely to be one subunit of N‐NTD in the asymmetric unit.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Chen, I Ung" sort="Chen, I Ung" uniqKey="Chen I" first="I-Jung" last="Chen">I-Jung Chen</name><name sortKey="Chou, Chia Heng" sort="Chou, Chia Heng" uniqKey="Chou C" first="Chia-Cheng" last="Chou">Chia-Cheng Chou</name><name sortKey="Hou, Ming On" sort="Hou, Ming On" uniqKey="Hou M" first="Ming-Hon" last="Hou">Ming-Hon Hou</name><name sortKey="Kan, Lou Ing" sort="Kan, Lou Ing" uniqKey="Kan L" first="Lou-Sing" last="Kan">Lou-Sing Kan</name><name sortKey="Lee, Cheng Hung" sort="Lee, Cheng Hung" uniqKey="Lee C" first="Cheng-Chung" last="Lee">Cheng-Chung Lee</name><name sortKey="Liu, Chia Ing" sort="Liu, Chia Ing" uniqKey="Liu C" first="Chia-Ling" last="Liu">Chia-Ling Liu</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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